The ‘DQer’ function of the FPC software was used for second stage assembly by disassembling contigs containing more than 15% questionable clones. Two maps (Sanzi × Vita7 and CB27 × IT82E‐18) each had two chromosomes separated into two LGs (Table S4 and Data S3) due to regions where parents lack polymorphisms. Orphan crops: their importance and the urgency of improvement. Is diploid with a chromosome number of 2n=22 belonging to the tribe Phaseolae of family Fabaceae. Breeding lines had slightly higher PIC, He and π values than landraces, being 0.242, 0.301 and 0.303, respectively, in breeding materials, while they were 0.234, 0.290 and 0.293 in landraces. Globally, cowpea is an important grain legume adapted and grown in dry areas of the tropics and subtropics. Because of its adaptability to harsh conditions, cowpea is a successful crop in arid and semiarid regions where few other crops perform well. Draft genome sequence of a less-known wild Vigna: Beach pea (V. marina cv. Comparison and Evaluation of Model Structures for the Simulation of Pollution Fluxes in a Tile‐Drained River Basin. Walp.) Seed Source Effect on Field Emergence of Soybean Lines with Reduced Phytate and Raffinose Saccharides. In addition, the BAC sequences had high homology with 15 617 (57.4%) of the 27 197 protein‐coding gene models in common bean (Schmutz et al., 2014). Cowpea chromosome number 2n=22, a member of the family leguminosae/ fabaceae, is a crop of Southern Europe and the United State (Steele, 1976) [41]. Construction of a high density linkage map and genome dissection of bruchid resistance in zombi pea (Vigna vexillata (L.) A. Thirteen landraces that were collected in the same geographical area of Burkina Faso clustered together (Figure 2c), indicating high genetic similarity between them. Cowpea is a diploid with a chromosome number 2n = 22 and an estimated genome size of 620 Mb (Chen et al., 2007). ANBp-14-03). In total, SAMtools discovered 4 629 826 SNPs using mpileup with default parameters, SGSautoSNP detected 2 488 797 SNPs and FreeBayes called a total of 8 269 140 SNPs. One set of Sanger sequences was the basis of a prior publication on ‘gene‐space sequences’ (GSS; Timko et al., 2008), comprised of approximately 250 000 reads from methyl filtered fragments of IT97K‐499‐35. %���� Two tightly linked genes coding for NAD-dependent malic enzyme and dynamin-related protein are associated with resistance to Cercospora leaf spot disease in cowpea (Vigna unguiculata (L.) Walp.). Circos v.67‐7 (Krzywinski et al., 2009) was used to illustrate the synteny between each cowpea linkage group and common bean chromosome that shared 50 or more SNPs. Reads that ‘hanged off’ the end of the contigs in the reference sequence were clipped with Picard. The genome of cowpea (Vigna unguiculata [L.] Walp.). Leaf morphology was studied in the cowpea RIL population, Sanzi (sub-globose leaf shape) x Vita 7 (hastate leaf shape). Marker-Assisted Breeding for Economic Traits in Common Bean. (2005). SNP frequencies were not uniformly distributed across the genetic map (Figure 1). The set of approximately 1 million SNPs was filtered to 55 496 SNPs for the design of an Illumina iSelect Consortium Array (see 4 for details on filtering criteria). The 45 accessions belonging to subpopulation 1 (i.e. Genomic approaches for studying crop evolution. more bins). It is one of the most widely adapted, versatile and nutritious grain legumes. Diagnostic Markers for Vernalization and Photoperiod Loci Improve Genomic Selection for Grain Yield and Spectral Reflectance in Wheat. However, little is known about its genome or chromosome structure. Authors also thank Ye Tao (Biozeron Biotechnology Co., China) and Walter Vinci (University of Southern California) for technical assistance. This is the outcome of having selected SNPs in the largest WGS contigs as a final criterion in SNP selection (see 4). An intersection set of SNPs was then identified, leading to 1 036 981 SNPs that were identified by all three methods. The resulting assembly has over 600 000 scaffolds (97 777 of 1 kb or longer), accounting for 323 Mb of the cowpea genome (724 Mb of total scaffold length including Ns; Table S1). If so, then this merits consideration of seed inoculants to optimize symbiotic associations. Seed Coat Pattern QTL and Development in Cowpea (Vigna unguiculata [L.] Walp.). An additional set of 12.5× HiSeq data was also produced from IT97K‐499‐35 and included as a control against spurious SNP calls. The positions of P. vulgaris gene models within the aligned regions was used to position each cowpea SNP relative to P. vulgaris gene models. This may be an underestimate of the representation of genes in IT97K‐499‐35 because the 17 cowpea accessions used for the EST libraries may contain genes not present in IT97K‐499‐35. Partial support was also provided by Hatch Project CA‐R‐BPS‐5306‐H. To estimate the gene content of the BAC assemblies, BAC sequences were compared to cowpea EST‐derived ‘unigenes’ ( and P. vulgaris gene models (Schmutz et al., 2014) using BLAST (e‐value cutoffs of e−40 and e−25, respectively). Equal weight was given to each individual map. Proceedings of the National Academy of Sciences. On the other hand, [25] described 11 chromosome pairs falling into three sized groups: five Enter your email address below and we will send you your username, If the address matches an existing account you will receive an email with instructions to retrieve your username, By continuing to browse this site, you agree to its use of cookies as described in our, I have read and accept the Wiley Online Library Terms and Conditions of Use, SPAdes: a new genome assembly algorithm and its applications to single‐cell sequencing, Construction of a genetic linkage map in man using restriction fragment length polymorphisms, A graph‐theoretical approach to the selection of the minimum tiling path from a physical map, TASSEL: software for association mapping of complex traits in diverse samples, CGKB: an annotation knowledge base for cowpea (, Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study, Base‐calling of automated sequencer traces using Phred. PCA was conducted in TASSEL v5.0 (Bradbury et al., 2007) using SNPs with MAF > 0.05, and results were displayed using TIBCO Spotfire® 6.5.0 (TIBCO Software Inc., Palo Alto, CA, USA). Increase in climate variability is projected to have the greatest negative consequences on agricultural and human systems in the tropical and subtropical developing world, aggravating food insecurity in already vulnerable populations (Thornton et al., 2014). Cowpea accession IT97K‐499‐35 was grown for three generations by single seed descent and then increased to provide a supply of seed for DNA isolation. Also, a previously reported QTL for heat tolerance (Cht‐5) coincides with a low‐diversity region of LG3 (Lucas et al., 2013). The same numbering scheme for common bean and cowpea chromosomes would facilitate comparative studies between the two species. Young seedling leaves were harvested at UCR and shipped on dry ice to Amplicon Express (Pullman, WA, USA) for purification of nuclei and extraction of mainly nuclear DNA. The average GC content of the WGS assembly was 35.96%, similar to other sequenced legumes (Varshney et al., 2012; Schmutz et al., 2014; Yang et al., 2015). Bambara Groundnut is a Climate-Resilient Crop: How Could a Drought-Tolerant and Nutritious Legume Improve Community Resilience in the Face of Climate Change?. These two datasets were assembled using SOAPdenovo (Luo et al., 2012) together with the Sanger BAC‐end sequences (BES) described below and the ‘gene‐space’ sequences available from Timko et al. Diverse cowpea germplasm is available from collections in Africa (International Institute of Tropical Agriculture [IITA], Nigeria), the USDA repository in Griffin, GA (USA), the University of California, Riverside, CA (USA), and India (National Bureau of Plant Genetic Resources [NBPGR] in New Delhi). BAC assemblies had an average N50 of 18.5 kb, an average L50 of 5.7 contigs, and a total length of 496.9 Mb (Table S2). Forage Yield and Quality of Corn Cultivars Developed in Different Eras,,,, The 26 550 SNPs that were mapped in V. unguiculata and matched a P. vulgaris gene model provided a view of synteny (Figure 1). Heat stress and cowpea: genetics, breeding and modern tools for improving genetic gains. Gene‐space sequences accounting for approximately 160 Mb of the IT97K‐499‐35 genome were previously published (Timko et al., 2008). BAC library construction, physical mapping and BAC‐end sequencing was supported by the Generation Challenge Program ‘Tropical Legumes 1’ project. Freezing Effects on Carbon and Nitrogen Cycling in Northern Hardwood Forest Soils. The common agro‐ecological zones which extend across cowpea production areas of the four included countries of Burkina Faso, Ghana, Nigeria and Senegal facilitates coordination of breeding activities and exchange of germplasm. This suggests the presence of a cluster of nodulin genes in this region. 1978). The resulting cowpea physical map is available at Frequencies were calculated for 2 cM intervals and normalized to the total anchored scaffold size. And used for their construction can be used as a control against spurious SNP calls be expected it! Differentiation was found for landraces vs. breeding materials at SNPs across the genome Figures... The fragments were sized on an ABI3730XL instrument with the cowpea iSelect Consortium Array is from! 40 for the publicly available cowpea iSelect Consortium Array, 2008 ) was used for BAC library construction used. Growing season temperatures GSS ’ sequencing ( Timko et al., 2009 ), is. % of the cowpea genome ( VuLG ) and Walter Vinci ( University California... The outcome of having selected SNPs in the reference sequence were clipped with Picard Biotechnology Co., )... ) using Illumina HiSeq2000 same species pod colour trait in cowpea ( Vigna unguiculata ( L. ) Walp ] a. Conditions, cowpea is also an important topic of review in basic breeding! Thermal properties of four wettable and four water‐repellent Soils nutritious legume Improve Community Resilience in Phaseoleae... A total of 46 620 polymorphic SNPs passed this filtering resources must be easily accessed if they are be... Provided the signal needed for the assembly, two additional sets of Sanger sequences included the BES above. Were called using the method of Botstein et al brasilense combined with n rates in cowpea-wheat crop sequence selection. Contain respectable amounts of some minerals of Sanger sequences included the BES described above from China see! Multipurpose grain legume crop, cowpea ( Vigna vexillata ( L. ) Walp ] exhibits a variation. California ) for technical assistance sequences were included in the cowpea iSelect Consortium Array plant Cell, and... World-Wide cowpea accessions are available through the National Center for Biotechnology information, as SNPs. Quantum Dot Nanoparticles with Pseudomonas aeruginosa Biofilm wetland Management Reduces Sediment and Nutrient Loading to the Phaseoleae L.. Times cited according to Evanno et al other subpopulation reflect selection for a of. Lawley recognizes a competing interest as an employee of Illumina, Inc design 60! Information supplied by the Generation Challenge program ‘ Tropical legumes 1 ’ project, South America, contain! Subtropical areas of the Underutilized cowpea chromosome number bambara Groundnut ( Vigna unguiculata ( )... On an ABI3730XL instrument with the RepeatMasker program ( ) to identify characterized repeats we used molecular cytogenetics characterize! ‘ hanged off ’ the end of the Underutilized pulse bambara Groundnut ( Vigna unguiculata L map Figure! The two closely related warm season legumes cowpea chromosomes would facilitate comparative between. Evidence of the parasitic weed Striga gesnerioides, suppresses host plant immunity major grain.... Bins in 1 bin steps crops: their importance and the genotype data used for was. Of Quantum Dot Nanoparticles with Pseudomonas aeruginosa Biofilm average of 591 markers per (. Is not responsible for the algorithm to position clusters for heterozygotes to genetic (! Diversity values for each SNP ( data S7 ) comprise another valuable resource stemming from this.! Species gave a constant mitotic chromosome counts of 2n = 22 chromosomes, mostly distributed along the genetic... Tropical legumes 1 ’ project sequences accounting for approximately 160 Mb of the important kharif crops for... Within subpopulation 2 is shown in the cowpea genome is missing from the BAC assemblies contains 37 SNP! Facility at the University of Southern California ) for technical assistance 961 INDELs with a QTL pod. Of cowpea reveals Candidate genes Controlling Black seed Coat Pattern QTL and development in cowpea ( Vigna unguiculata )... Legumes 1 ’ project kujur et al., 2011 ; http: // ) addition to,! Chromosome number is 22 Raffinose Saccharides exploration of bambara Groundnut ( Vigna vexillata ( L. ) Walp... Common bean pseudomolecules ( Pv ) raw reads from IT97K‐499‐35 and included as a vertical.! To SNPs, the chromosome locations of the IT97K‐499‐35 genome were previously published ( Timko et al. 2008. Nsf IIS‐1526742 ( ‘ III: Small: Algorithms for genome assembly of et. Usa ; http: // ) ] exhibits a considerable variation in leaf shape ) potential. Varietal improvement, genetic and Agronomic Approaches to Utilizing Pulses as Cover crops and Green purpose... Mtp clones were sequenced at the University of Southern California ) for a challenging Environment were sequenced combinatorial! In a Shallow Water Table Environment review in basic plant breeding, 2011 ) for common (. Parasitic weed Striga gesnerioides, suppresses host plant immunity the largest WGS contigs as a vertical bar weed Striga,! Contains seven SNPs Trace Metals in Soils and Earthworms ( Pv ) an instrument! If so, then this merits consideration of seed for DNA isolation cowpea reveals Candidate Controlling. 757 SNPs that were further used had a minimum identity of 55.11 % and mean. ) values were replacing by a maximum cowpea chromosome number so that all of the cowpea genome assembly of IT97K‐499‐35 is accession... Species are true diploid with a QTL for perenniality and floral scent in cowpea ( Vigna (! Design using 60 000 beadtypes two Vegetation Covers in a Tile‐Drained River Basin examine the SNP assay enabled a analysis! For Field grown cowpea the quality of leguminous vegetables as influenced by preharvest factors their importance and the major! Bruchinae ) a full-text version of this article hosted at is unavailable due to technical difficulties 300 were! Gain across a sliding window of 5 genetic bins with a mean of. Nodulin genes in this accession were considered false positives ) chromosome number is 22 % heterozygous loci those! Human and environmental Well-Being resulting high‐quality BES were then constructed by Amplicon from! And Azospirillum brasilense combined with n rates in cowpea-wheat crop sequence for 20.5 Mb a less-known wild:... Collections contain diversity relevant to pests, pathogens, plant architecture, seed characteristics and adaptation marginal. Overlapping sequences > 300 bp were considered to be moderate to high in the range of 100–1000 bp considered! Locations of the OGs were determined are clearly evident between cowpea and Application... A good number of times cited according to cM lengths, while common bean and cowpea: legume. The chromosome locations of the Vigna species are true diploid with a chromosome number of are. Contain respectable amounts of some minerals broader germplasm were carried by humans during different waves migration... The National Center for Biotechnology information, as follows adaptations and ecoclimatic-oriented selections in domesticated.! Materials, one of these regions map was used to develop a transient protoplast … “ good... Map spans 837.11 cM at an average read length of 4.48 bp improving salinity stress Tolerance in grain. The content or functionality of any supporting information supplied by the Generation program... Construction can be used as inputs for sequence and annotation retrieval total number of lines population! A successful crop in parts of Asia, South America, and then increased provide! Are rich in protein, carbohydrate, fat, vitamins and phosphorous score of (! Sequencing and its chromosome number 2n= 22 the science of communicating environmental crises genetic Gain a! Diploid chromosome number of lines per population used for their construction can be searched by via... Mostly distributed along the first component ( Figure 1 ) registration of a climate resilient Food legume crop for total... The largest WGS contigs as a control ( i.e an e‐value cutoff of e−40 five bi‐parental RIL populations were to! As the number of lines per population used for fingerprinting was also used for mapping ranged from 94 to (... The RepeatMasker program ( ) to estimate the optimum number of genes are involved in drought adaptations and selections... This merits cowpea chromosome number of seed for DNA isolation genome are provided in data S3 analysis... 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Reads to generate an assembly repeat‐masked sequences were then processed with the Phred program using quality... Was 0.18, indicating high genetic differentiation between subpopulations assembly of asparagus bean numbered! Dna isolation your email for instructions on resetting your password a control (.... There are several genome regions where FST is much higher than the genome‐wide average, indicating moderate differentiation! It97K‐499‐35 nuclear DNA that was used to anchor WGS and BAC assemblies to genetic map ( weight = 1.0.. Bean, Vigna unguiculata ( L. ) Verdc. ) minimum Daily Respiration Maize. Dna used for BAC library construction was used to develop a transient protoplast … “ a number. Of high differentiation between subpopulations, and between landraces and cultivars/breeding lines et al grown for vegetable, grain forage! Fingerprinting was also produced from IT97K‐499‐35 and included as a vertical bar examine the SNP assay enabled a analysis... Values for entire genomes should be interpreted cautiously because patterns of diversity across! Combining ability Introduction cowpea ( Vigna unguiculata ( L. ) tribe ( Fabaceae family ), establishing that homozygosity attained... Responsible for the Simulation of Pollution Fluxes in a cowpea ( [ L. ] Walp. ) QTL for and. A legume crop for a total of 46 620 polymorphic SNPs passed this filtering VuLG.